本文是来自 Dulwich College Beijing 的 Mika 同学的投稿。2019年圣诞期间，她参加了极萨学院开设的纳米药物递送（靶向药）系统研究定制科研项目，并对这次完整的科研实践进行了回顾与总结，现分享给大家。也欢迎更多极萨学院的学员向我们投稿，分享科学带给你的智慧与快乐。

　　▲The HeLa cells that we cultured under a light microscope

　　▲HeLa cell thawing in 37℃ water bath performed under one minute

　　▲Taking out 20ml of cholesterol from the container. We had to measure it on an electronic precision digital balance to ensure preciseness

　　▲This is a picture showing the HeLa cells before the GFP knockout, under the fluorescence microscope. The cell membranes are exhibiting a green glow.

　　▲This is a picture showing the HeLa cells after the GFP knockout, under the fluorescence microscope. There has been a significant reduction in green cell membrane glow.

　　▲The y-axis is the percentage comparison of our target knock-out gene to the reference gene GAPDH. The x-axis are the results.

　　▲The y-axis represents the survival percentage of the HeLa cells and the x-axis are the results. D5A14 is the sample name of the cationic lipid used in our experiment.

　　The concept here is that the MTT solution will turn mitochondrial succinate dehydrogenase purple if the cells are alive, and no colour change would occur if the cells are dead. Looking at the 96 pore hole board, the cells turned a dark purple colour, indicating that most cells still survived. This is not the intended result as we wanted to kill the cancer cells; this outcome may be the consequence of the different sized nanoparticles used to deliver RNAi, reducing the endocytosis of nanoparticles, thus, the release of the RNAi. Overall, I realized that conducting experiments ourselves was than watching others do it. The different mediums and buffers that we had to add was a challenge to our memories. But thanks to our frantic note taking, we were able to keep up with the procedures. On the whole, research and experiments are precise and arduous work that requires high concentration to achieve. Us as beginners got a taste of this field and look forward to future opportunities. In this world of science, we seek to unravel the issues that burden the heart of humanity.

　　极萨学院是中国第一所由民间力量发起的少年科学院，由国内知名的青少年科学研究及学术项目提供平台爱博物发起，旨在为中国青少年提供高质量的科学实践平台。 爱博物先后获得了华大基因、好未来教育产业基金的投资，凭借强大的科学与资本背景，极萨学院结集了全球数十位多学科一线科学家，以及分布在包括中国、美国、丹麦、挪威、新加坡、澳大利亚等多个国家的数十所大学及科研机构的联合实验室资源，致力于为学员提供高端STEM进阶学术项目与精英培养计划。 力求培养中国少年的科学精神，为中国乃至全球科技人才储备力量。